Transformation with green fluorescent proteinProveedor: Edvotek
EDVO223/AP08EA 138 EUREDVO223/AP08
Transformation with green fluorescent protein
Materiales educativos Materiales educativos de biología Actividades de aprendizaje de microbiología
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
- For 10 lab groups
- Plating 5 minutes; incubation overnight; transformation efficiency 15 minutes
Also required: 37 °C incubator, water bath, longwave UV lamp.
Información de suministro: Kit includes instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, loops and microtubes.