Experimentation kits, southern blot analysis

Proveedor: EDVOTEK EUROPE LTD
EDVO207EA 185 EUR
EDVO207
Experimentation kits, southern blot analysis
Materiales educativos Materiales educativos de biología Physiology and Health Learning Activities
This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

  • For 5 lab groups
  • Electrophoresis 45 minutes, blotting overnight, staining and destaining 10 minutes

Also required: Electrophoresis tank and power supply, 65 °C water batch, DNA visualisatoin system, staining net and tray, automatic micropipettes, lab glassware, microwave oven, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, and forceps.

Información de suministro: Kit includes instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose, electrophoresis buffer, pipettes, 5 precut nylon membranes, 5 precut blotting filter papers, and Blue-Blot DNA stain.
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