GelRed® Prestain Plus 6X DNA loading dyeProveedor: Biotium
- 6X loading dye includes GelRed® DNA stain
- One-step gel loading and DNA staining
- Improved formulation to minimise DNA migration issues
- Two visible blue tracking dyes that run at ~1,5 kb and ~200 bp in 1% agarose
- Highly sensitive, non-mutagenic red fluorescent DNA dye
GelRed® Prestain Plus 6X DNA loading dye contains density agents, tracking dyes, and GelRed® dye. The 6X prestain Loading Dye is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting or after electrophoresis. The loading dye contains two blue electrophoresis tracking dyes that run at approximately 1,5 kb and 200 bp in a 1% agarose gel.
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. It is efficiently removed from DNA by gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
How Safe is Your Gel Stain?
Many so-called 'safe' DNA dyes like SYBR® Safe, Midori Green, GreenSafe, SafeView™, and RedSafe™ not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic. Unlike these dyes, GelRed® is cell membrane-impermeant, so it cannot enter living cells to interact with their DNA.