PCR polishing kit increases blunt-end cloning efficiencies associated with PCR-generated fragments.
- Generates blunt-ended DNA fragments in 30 minutes
- More efficient than Klenow polishing
- Optimizes blunt-end cloning efficiencies
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilising blunt-ended ligation.