FURA-2 pentapotassium salt ≥95% (by HPLC) fluorescent indicator, for calcium determination

Proveedor: Biotium

50031.EA 259 EUR
50031.
FURA-2 pentapotassium salt ≥95% (by HPLC) fluorescent indicator, for calcium determination
FURA-2 pentapotassium salt

Fura-2, pentapotassium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading.


  • Kd: 145 nM (22°C in pH 7.2 buffer)
  • λEx/λEm (no Ca2+) = 363/512 nm; λEx/λEm (high Ca2+) = 335/505 nm
  • ε (363 nm, no Ca2+) = 27,000 M-1cm-1; ε (335 nm, high Ca2+) = 35,000 M-1cm-1
  • Cell permeability: Membrane impermeant
  • Light yellow solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution


Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.

Formula: C₂₉H₂₂K₅N₃O₁₄
MW: 832 g/mol
Temperatura de almacenaje: Nevera
Núm. CAS: 113694-64-7

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