MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand.
Upon specific binding via MC192 to the cells expressing p75NTR, saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75NTR-expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo, MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75NTR receptors. The average molecular weight of the MC192-saporin conjugate is approximately 210 kDa, and its purity >90% by non-reducing SDS-PAGE. The conjugate is routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Precaución: Note: Only qualified personnel should handle this product. MC192 antibody does not bind to human p75NTR receptor, however, standard laboratory protective measures should be followed, including wearing lab coat, gloves and safety glasses. Avoid contact with open wounds and practice good laboratory technique to avoid auto-exposure/auto-injection! Avoid contact of conjugate with reducing agents (eg., DTT, beta-mercaptoethanol) as this will destroy the antibody-saporin conjugate.
Type: Primary
Antigen: NGFR
Clonality: Monoclonal
Clone: MC192
Conjugation: Saporin
Epitope: Extracellular
Host: Mouse
Isotype: IgG1
Reactivity: Rat
