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Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Proveedor: Biotium

NucView™488 RedDot™2
30072.EA 532 EUR
30072.
Apoptosis and necrosis assay kit, NucView™488 RedDot™2
Ensayos Ensayos celulares
This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

  • NucView® 488 caspase-3 assay kit for live cells with Ac-DEVD-CHO caspase-3 inhibitor for detecting caspase-3 activity within live cells in real time
  • NucView® 488 and CF™dye (594, 640R and Texas REd) conjugated Annexin V provides two apoptosis readouts, caspase-3 activity and phosphatidylserine (PS) translocation
  • NucView® 488 and MitoView™633 kit allows profiling apoptotic cells simultaneously with changes in mitochondrial membrane potential
  • NucView® 488 and RedDot™2 assay kit allows staining of necrotic and late apoptotic cells that have compromised plasma membrane integrity

In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView™488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView™488 Caspase-3 Substrate allows detection caspase-3/7 activity and visualisation of morphological changes in the nucleus during apoptosis.

RedDot™2 is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. RedDot™2 has far-red emission at 695 nm, well-separated from the green fluorescence of NucView™488. The excitation maximum of RedDot™2 is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.

The dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate and Annexin V provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation. The kit contains sufficient reagents for 50 flow cytometry assays (200 µl assay volume). The number of fluorescence microscopy assays that can be performed with the kit may vary based on the size of culture vessel and staining volume used.

The NucView® 488 and MitoView™ 633 Apoptosis assay kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

The NucView® 488 and RedDot™2 apoptosis and necrosis assay kit provide a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry. While NucView®488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot™ is not recommended because it can result in increased background staining of healthy cells.

Precaución: While NucView™488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot™2 is not recommended because it can result in increased background staining of healthy cells.
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