MicroLink™ Peptide Coupling Kit enables simple and efficient covalent immobilisation of sulphhydryl-containing peptides, proteins and other ligands to a gel support for use in small-scale affinity purification procedures.
- Ideal for coupling small amounts (25 to 250 µg) of sulphhydryl-containing peptide or protein for purification of specific antibodies from crude serum
- High-performance affinity resin, polyacrylamide-based, for specific reaction to sulfhydryl groups
- Immobilisation efficiency >80%
- Fast and easy to use – perform wash and elution steps using a microcentrifuge
- Prepared peptide resin can be used several times with no significant loss of capacity
MicroLink™ Peptide Coupling Kit uses UltraLink™ Iodoacetyl gel that reacts specifically with free sulphhydryl's to form a stable thioether linkage. The support contains a 15-atom spacer that reduces steric hindrance, making binding interactions with the coupled molecule efficient. Average coupling efficiencies are greater than 80%, although efficiency is related to sulphhydryl content and is variable. This kit is ideal for coupling small quantities of sulphhydryl-containing peptide for subsequent antibody purification.
Información de suministro: Kit contains UltraLink™ Iodoacetyl spin columns (0,1 ml, 10 columns), wash solution (25 ml), L-Cysteine.HCl (100 mg), Coupling buffer (100 ml), IgG Elution buffer (50 ml), PBS pack (makes 500 ml) and collection tubes (200 tubes).