The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.
- Spin columns eliminate resin loss and provide efficient separation of solutions
- Immunoprecipitate (IP) in less than one hour
- Recover antigen without harsh detergents or reducing agents
- Purify target protein from crude lysate in four simple steps
This kit involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.
Información de suministro: The kit includes protein A/G plus agarose, 0,55 ml; IP lysis/wash buffer, 2×50 ml; conditioning buffer (100X), 5 ml; tris-buffered saline (20X), 25 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; control agarose resin, 2 ml; spin columns and collection tubes.
