X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)

Proveedor: G Biosciences

Sinónimos: BCIG

RC-213 RC-212
X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)
X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galactopiranósido)

X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Formula: C₁₄H₁₅BrClNO₆
MW: 408,63 g/mol
Número MDL: MFCD00005666
Núm. CAS: 7240-90-6

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