C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
The antibody reacts specifically with human C-reactive protein (CRP) and likely reacts with C-reactive protein from mouse, rat, pig, and other mammalian species. CRP monomer runs as 21-25kDa in fully reduced westerns. CRP in native state is a homopentameric protein of 115-118 kDa in size non-reduced. Human serum that has been separated from whole blood and concentrated though a 3 kDa centrifugal concentrator can serve as a positive control. The antibody also detects human C-reactive protein in ELISA.
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Antigen: C-reactive Protein
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody