Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.
The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry.
Application Information:
WB, IHC/IF. Suggested dilution of at least 1:1,000 for IHC/IF. Dilutions of 1:10,000 or lower is recommended for WB. This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: DJ-1
Clonality: Monoclonal
Clone: 4H4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity:
![WB analysis of whole brain and cell lysates using mouse antibody to DJ1. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The blot was simultaneously probed with chicken antibody to vimentin (red), revealing a single band at about 50 kDa.](https://es.vwr.com/stibo/low_res/std.lang.all/70/61/26507061.jpg "WB analysis of whole brain and cell lysates using mouse antibody to DJ1. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The blot was simultaneously probed with chicken antibody to vimentin (red), revealing a single band at about 50 kDa.")
![WB analysis of whole brain and cell lysates using mouse antibody to DJ1. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The blot was simultaneously probed with chicken antibody to vimentin (red), revealing a single band at about 50 kDa.](https://es.vwr.com/stibo/grid/std.lang.all/70/61/26507061.jpg)
