Cell proliferation Biotrak™ ELISA is designed as a precise, fast, and simple colorimetric assay. The technique is based upon the incorporation of the thymidine analog, 5'-bromo-2'-deoxyuridine into the DNA of proliferating cells cultured in a 96-well microplate.
- Non radioactive
- High level of correlation with the number of proliferating cells, with low mean variation
- As least as sensitive as ³H-thymidine incorporation
- Immunoassay can be performed in ∼3 hours
- Use of a multiwell ELISA reader allows a large number of samples to be processed simultaneously
Used in conjunction with RPN20, the reagent allows extended use of the kit where additional amounts of labeling reagent are required, such as in vivo studies.
The antibody contains a nuclease to produce single stranded DNA without the need for chemical denaturation. Cellular morphology is preserved allowing multiple labeling experiments to be carried out. The high-contrast stain produced has sensitivity comparable to autoradiographic methods.
Información para pedidos: Additional labeling reagent and antibody for use with the immunocytochemistry kit are available separately. NOTE: DAB component must be ordered separately.