- Digestion of protein spots in 96 well titre plate format
- Suitable for 2D gels stained with silver stain, Coomassie® dye, and other methods
The protein spots are first excised from the silver stained gel and transferred to a proteomic grade titre plate. Silver stained gel pieces are washed with SilverOUT™ to remove inhibitory silver ions. The proteins are then digested within the gel using a mass spectrometry grade trypsin and a supplied trypsin digestion buffer.
The digested peptides are extracted with G-Biosciences' Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without any subsequent treatments or cleaning procedures.
Información de suministro: InGel™ Array kit is supplied with reagents sufficient for up to 500 protein spots. The kit includes SilverOUT™ for the removal of silver ions, trypsin digestion buffer for optimal trypsin activity, Pep-Extract™ for high level peptide extraction, and the InGel™ Array titre plates and caps. MSG-Trypsin™ (Mass Spectrometry Grade) trypsin, which increases stability, is also available but sold separately.