Substrate for Western blotting, ECF™

Proveedor: Cytiva
RPN5785EA 258 EUR
Substrate for Western blotting, ECF™
Reactivos para electroforesis Western Blotting Reagents
  • A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
  • For use in western blotting, dot, and slot blotting applications
  • Sensitivity comparable to chemiluminescence
  • The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminates need for darkroom and film

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

Información de suministro: The following reagents are provided, sufficient for detecting 2500 cm² membrane: ECF Substrate, 60 ml, and dilution buffer.
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