Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of native and recombinant proteins
- BioProcess medium supported for industrial applications and well-established in approved processes
- Stable in commonly used aqueous buffers - 1 M NaOH, 20% ethanol, 8 M guanidine hydrochloride, 8 M urea, 0.01 M HCl, non-ionic detergents, acetate buffer pH 4
Chelating Sepharose Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. Chelating Sepharose Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.